For the convenience of analyzing influence of fiber orientation and concrete interlayer on mechanical properties of layered steel fiber-reinforced concrete (LSFRC), firstly, layered steel fiber-reinforced concrete samples with three kinds of fiber content of 0.5%~1.5% are tested for compressive strength, shear strength and flexural tensile strength experiment respectively. And then, fine models of cubic and prismatic LSFRC samples are established by using finite element to analyze effects of fiber content, fiber orientation and the interlayer between concrete layers. The experimental results show that LSFRC exhibit a maximum increment of 58% in flexural strength range. The numerical analysis indicates that the interlayer maximum shear stress is reduced by 10.8% in LSFRC prismatic samples, and the deflection at the bottom of prismatic samples is decreased by 17.3%. The study demonstrates that the bonding states between inter-layers have a significant effect on the loading of specimens. The incorporation of steel fibers is the result of a local stress concentration and an integral stress dispersion in the part of samples, whereas the steel fibers restrain the deformation of zone, improving the bending resistance of concrete.