Aimed at the problem that temperature affects the load measurement accuracy of composite structure by the strain method, a load measurement method is proposed in consideration of the effect of temperature on composite structure. In this paper, taking shear force measurement of carbon fiber composite in horizontal tail box at a certain type of aircraft as an example, tests are to be made. Firstly, the load calibration tests under different temperature environments are designed and completed. Secondly, the multiple linear regression method is used to establish a load measurement model at room temperature, and the load model is checked under different temperature conditions. Finally, the load model at room temperature is modified and verified in consideration of the effect of temperature. The results show that the method in this paper is feasible and the result is accurate