In order to improve the flow control ability of plasma actuator, further optimization of the endwall actuation layouts is needed. Different endwall millisecond pulse plasma actuation layouts are carried out on lowspeed cascade wind tunnel. The experiment results show that the influence of the lateral flow on the endwall is greater than the axial flow. The layouts of plasma actuators are of vital importance to the flow control effects. The tangential plasma actuation layout is optimal for controlling the corner separation and total pressure loss coefficient could be reduced by 11.8% at 50% span. Nevertheless, with the change of the angle of attack, the layout of the actuator is no longer being tangential to the separation line on the endwall, weakening the flow control effect. An appropriate actuation layout is necessary for controlling the scope of the attack angle requirement. The appropriate increase of the plasma actuators could effectively promote the mixing of jet and near wall airflow and improve the flow control effect.