Researches on Transform Domain Communication System (TDCS) are almost confined to theoretical simulation and corroboration, and rarely involve hardware platform. In this paper, firstly, the fundamental and key techniques of TDCS are analyzed, the working principle of USRP2 is introduced; and then a dynamic platform of TDCS based on USRP2 is designed, the dynamic simulation module library of TDCS is compiled by Level-2 Matlab S-Function. Finally, the communication system is set up in the environment of Matlab/Simulink. Signal transmission and reception in actual wireless channel are tested through two USRP2. The results indicate that the TDCS system designed in this paper based on USRP2 can accurately realized signal transmission and reception in actual wireless channel, and has better data transmission error rate. This paper is of a certain value to the practical application of Cognitive Radio technology.