A target assignment method, interaction mechanism of the genetic algorithm (GA) combined with the discrete particle swarm optimization (DPSO) is put forward based on cultural algorithm (CA). According to the characteristics of MAV/UAV target assignment problem, the interaction mechanism between GA and DPSO is established based on the basic framework of CA. The proposed algorithm improves the search ability and overcomes easily to run into partial optimization. The algorithm can solve the MAV/UAV cooperation mission assignment problem effectively. The simulation results show that the proposed algorithm is better than GA and DPSO. The algorithm has a good convergence rate and can find the best assignment scheme fleetly.