his paper applies finite element approach to establishing multi-holed plate finite element mode and analyzing the stress of wing longeron web with several radius holes or openings. And then it determines the general stress concentration factor considering the difference of apertures and the spacing between holes. Furthermore, the paper translates the realistic centrobaric loading spectra into the spectra of wing spar web on the basis of test data and modifies curves of δmax-N and εeq-Nf under low wing loading spectra. And then, it applies Miner's rule and local stress-strain approach respectively to calculating fatigue life of the plate and analyzes the results of the two approaches.