It is of interest to study the plasma flow control on the ground wind tunnels. In order to explore the key technology of plasma flow control in application, the flight verification research on low speed UAV plasma flow control is carried out. Firstly, both these UAV test flight test system and the flight test plan are designed. On these grounds, the excitation characteristics test and the ground wind tunnel experimental study are carried out. The discharge characteristics of the plasma excitation are tested, and the harmonic pulse flow control to the Viper Jet UAV stall separation is verified in the wind tunnel. Then, the Viper Jet UAV plasma flow control flight verification is carried out. The results show that the ACDBD actuation can effectively reduce the takeoff distance and the landing distance for Viper Jet UAV.